> 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CLMF, CLMF2, IL-12A, IL-12B, IL12, IL12 p70, IMD28, IMD29, NFSK, NKSF1, NKSF2, P35, IL-12 subunit p35, IL12A, interleukin 12,Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
分子量
34.7(IL-12B)/25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.