TNF alpha Protein (AA 77-233)
-
- 抗原 See all TNF alpha 蛋白
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 77-233
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
-
Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.
- Top Product
- Discover our top product TNF alpha 蛋白
-
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha 宿主: 兔 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (GST tag)TNF alpha 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Tumor Necrosis Factor alpha (TNF alpha) protein (His tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)TNF alpha 宿主: 犬 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Biological activity: The ED50 is 0.020-0.10 ng/ml, corresponding to a specific activity of 1-5 x 107 units/mg, as determined by a dose dependent stimulation of L929 cells treated with actinomycin D.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
-
- 抗原
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 别名
- TNF-alpha (TNF alpha 产品)
- 别名
- DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
- 背景
- TNF-α is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-α is released from cells by proteolysis of the integral membrane precursor form of TNF-α. TNF-α binding to some TNF-α receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-α is involved in the inflammatory response.
- 分子量
- The 157 amino acid recombinant protein has a predicted molecular mass of 17,352 Da. The DTT-reduced protein and non-reduced protein migrate at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Val.
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
-
