NFKB1 ELISA 试剂盒
NFKB1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all NFKB1 ELISA试剂盒
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NFKB1
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))
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适用
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人
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Human NFKB1 ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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特异性
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This ELISA antibody pair recognizes Human NFKB1.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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储存条件
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4 °C
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有效期
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6 months
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抗原
See all NFKB1 ELISA试剂盒
-
NFKB1
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))
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别名
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NFKB1 (NFKB1 产品)
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别名
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GKLP ELISA Kit, NKTL ELISA Kit, NTKL ELISA Kit, P105 ELISA Kit, TAPK ELISA Kit, TEIF ELISA Kit, TRAP ELISA Kit, EBP-1 ELISA Kit, KBF1 ELISA Kit, NF-kB1 ELISA Kit, NF-kappa-B ELISA Kit, NF-kappaB ELISA Kit, NFKB-p105 ELISA Kit, NFKB-p50 ELISA Kit, NFkappaB ELISA Kit, p105 ELISA Kit, p50 ELISA Kit, NF-kB ELISA Kit, NF-KB1 ELISA Kit, ebp-1 ELISA Kit, kbf1 ELISA Kit, nf-kappa-b ELISA Kit, nf-kappaB ELISA Kit, nfkb-p105 ELISA Kit, nfkb-p50 ELISA Kit, NF-kappaB1 ELISA Kit, p50/p105 ELISA Kit, BICDR-1 ELISA Kit, bicdr1 ELISA Kit, ccdc64 ELISA Kit, SCY1 like pseudokinase 1 ELISA Kit, nuclear factor kappa B subunit 1 ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 L homeolog ELISA Kit, nuclear factor NF-kappa-B p105 subunit ELISA Kit, nucleoporin 107 ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit, SCYL1 ELISA Kit, NFKB1 ELISA Kit, Nfkb1 ELISA Kit, nfkb1.L ELISA Kit, CpipJ_CPIJ012236 ELISA Kit, Nup107 ELISA Kit, nfkb1 ELISA Kit
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途径
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p53 Pathway, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins
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