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IL1A ELISA 试剂盒

IL1A 适用: 小鼠 Colorimetric Cell Culture Cells, Plasma, Serum
产品编号 ABIN2648756
发货至: 中国
  • 抗原 See all IL1A ELISA试剂盒
    IL1A (Interleukin 1 alpha (IL1A))
    适用
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    小鼠
    检测方法
    Colorimetric
    应用范围
    ELISA
    原理
    Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 1 Alpha (IL-1α) concentrations in cell culture supernates, serum, and plasma.
    样品类型
    Serum, Plasma, Cell Culture Cells
    Analytical Method
    Quantitative
    灵敏度
    1 pg/ml
    产品特性
    Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    样本量
    100 μL
    实验时间
    3.5 h
    板类型
    Pre-coated
    实验流程
    Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1α is added to the wells and binds to the combination of capture antibody-IL-1α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1α standard dilutions and IL-1α sample concentration determined.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all IL1A ELISA试剂盒
    IL1A (Interleukin 1 alpha (IL1A))
    别名
    IL-1a (IL1A 产品)
    别名
    IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit
    途径
    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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