IL1A ELISA 试剂盒
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北京 101111
Quick Overview for IL1A ELISA 试剂盒 (ABIN411292)
抗原
See all IL1A ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 4.69 pg/mL
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原理
- Sandwich Rat IL-1 Alpha ELISA Kit to quantitate Rat Il1a in cell culture supernatants and serum.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S115-S270Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 0.5 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 山羊 Colorimetric Sandwich ELISA 0.781 pg/mL - 50 pg/mL Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 猴 Colorimetric Sandwich ELISA 3.906 pg/mL - 250 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: Hamster Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 马 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.1%, 5.3%, 7.4%
Inter-Assay Precision (CV%): 7.8%, 6%, 9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).
: "Cytokine Attenuation and Free Radical Scavenging Activity of a New Flavanone7,4'-Dihydroxy-3″,3″-Dimethyl -(5,6-Pyrano-2″-One)- 8- (3‴,3‴-Dimethyl Allyl)- Isolated from Mallotus philippensis: ..." in: PLoS ONE, Vol. 11, Issue 12, pp. e0167294, (2017) (PubMed).
: "Anti-inflammatory effect of Fumaria parviflora leaves based on TNF-α, IL-1, IL-6 and antioxidant potential." in: Avicenna journal of phytomedicine, Vol. 7, Issue 1, pp. 37-45, (2017) (PubMed).
: "Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 6063475, (2016) (PubMed).
: "Anti-inflammatory activity of roots of Cichorium intybus due to its inhibitory effect on various cytokines and antioxidant activity." in: Ancient science of life, Vol. 34, Issue 1, pp. 44-9, (2015) (PubMed).
: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." in: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, (2014) (PubMed).
: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).
: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).
: "Brain microglia were activated in sporadic CJD but almost unchanged in fatal familial insomnia and G114V genetic CJD." in: Virology journal, Vol. 10, pp. 216, (2014) (PubMed).
: "Baicalin attenuates brain edema in a rat model of intracerebral hemorrhage." in: Inflammation, Vol. 37, Issue 1, pp. 107-15, (2014) (PubMed).
: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." in: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, (2013) (PubMed).
: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." in: Clinical & developmental immunology, Vol. 2012, pp. 192791, (2012) (PubMed).
: "[Present state and improvement of the methods of preventing ORL diseases in industrial workers]." in: Vestnik otorinolaringologii, Issue 2, pp. 3-8, (1985) (PubMed).
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- IL1A
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背景
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Background: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18 kDa.
Gene Full Name: interleukin 1 alpha
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基因ID
- 24493
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UniProt
- P16598
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原 See all IL1A ELISA试剂盒
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