POLD1 抗体 (AA 60-261)
(5 references)-
- 抗原 See all POLD1 抗体
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
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抗原表位
- AA 60-261
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This POLD1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 交叉反应
- 大鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human DNA Polymerase delta aa. 60-261
- 克隆位点
- 22-D Polymerase delta
- 亚型
- IgG1
- Top Product
- Discover our top product POLD1 Primary Antibody
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anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) antibody
POLD1 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (Center) antibodyPOLD1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 1-123), (Catalytic Subunit) antibodyPOLD1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 41-254) antibodyPOLD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 101-200) antibodyPOLD1 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) antibodyPOLD1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (C-Term) antibodyPOLD1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 710-739) antibodyPOLD1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB17790 unconjugated
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- 说明
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Related Products: ABIN967389, ABIN968537
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).
: "Characterization of the p125 subunit of human DNA polymerase delta and its deletion mutants. Interaction with cyclin-dependent kinase-cyclins." in: The Journal of biological chemistry, Vol. 273, Issue 16, pp. 9561-9, (1998) (PubMed).
: "Expression and characterization of the small subunit of human DNA polymerase delta." in: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 13013-8, (1997) (PubMed).
: "DNA polymerase delta is involved in the cellular response to UV damage in human cells." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 13748-51, (1994) (PubMed).
: "Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta." in: Nucleic acids research, Vol. 20, Issue 4, pp. 735-45, (1992) (PubMed).
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: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).
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- 抗原
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
- 别名
- DNA Polymerase delta (POLD1 产品)
- 别名
- CDC2 antibody, CRCS10 antibody, POLD antibody, 5949 antibody, CAA61369 antibody, CG5949 antibody, DNA Pol Delta antibody, Dm Poldelta antibody, Dmel\\CG5949 antibody, Poldelta antibody, dnapol delta antibody, l(3)72Ac antibody, cdc2 antibody, pold antibody, 125kDa antibody, Pol delta antibody, fla antibody, im:7139126 antibody, zgc:152688 antibody, DNA polymerase delta 1, catalytic subunit antibody, DNA-polymerase-delta antibody, polymerase (DNA directed), delta 1, catalytic subunit antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase delta antibody, POLD1 antibody, DNApol-delta antibody, pold1 antibody, LOC100456571 antibody, Pold1 antibody
- 背景
- Errors in DNA sequence result from environmental factors or are committed by DNA polymerases during replication. If unchecked, these errors might accumulate genetic damage such that the cell could no longer function. Thus, DNA repair processes involve mechanisms for the excision of damaged sequences and the resynthesis and ligation of the proper sequence. In mammalian cells, this proofreading function rests with DNA polymerase (pol) delta, a heterodimer of a 50kDa subunit, which stimulates pol delta activity in the presence of PCNA (proliferating cell nuclear antigen) and a 125kDa catalytic subunit. The catalytic subunit has 3' to 5' exonuclease activity which distinguishes pol delta from pol alpha and pol beta. Pol delta is also central to DNA replication where it functions in leading strand synthesis at the replication fork. The catalytic subunit is phosphorylated by G1 cyclin-dependent kinase-cyclin complexes and, via its N-terminal 249 amino acids, interacts with cdk2. However, phosphorylation has little or no effect on the activity of pol delta. Thus, DNA polymerase ä is essential for DNA replication and is unique in its ability to replace damaged sequences through the process of DNA excision repair.
- 分子量
- 125 kDa
- 途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA
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