POLD1 抗体 (C-Term)
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北京 101111
Quick Overview for POLD1 抗体 (C-Term) (ABIN6258118)
抗原
See all POLD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- POLD1 Antibody detects endogenous levels of total POLD1.
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预测反应
- Zebrafish,Bovine,Horse,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human POLD1, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) antibody
POLD1 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (Center) antibodyPOLD1 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 1-123), (Catalytic Subunit) antibodyPOLD1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 41-254) antibodyPOLD1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) antibodyKD Validated POLD1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3335 unconjugated Recombinant Antibody
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 101-200) antibodyPOLD1 适用: 人, 大鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) antibodyPOLD1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 710-739) antibodyPOLD1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB17790 unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
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别名
- POLD1
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背景
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Description: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196).
Gene: POLD1
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分子量
- 125 kDa
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基因ID
- 5424
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UniProt
- P28340
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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