POLD1 抗体 (Catalytic Subunit)
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- 抗原 See all POLD1 抗体
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
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抗原表位
- AA 1-123, Catalytic Subunit
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLD1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应
- 人
- 纯化方法
- >95%, Protein G purified
- 免疫原
- Recombinant Human DNA polymerase delta catalytic subunit protein (1-123AA)
- 亚型
- IgG
- Top Product
- Discover our top product POLD1 Primary Antibody
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anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) antibody
POLD1 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (Center) antibodyPOLD1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 41-254) antibodyPOLD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 101-200) antibodyPOLD1 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) antibodyPOLD1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (C-Term) antibodyPOLD1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1) (AA 710-739) antibodyPOLD1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB17790 unconjugated
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- 应用备注
- Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000,
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4 - 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- 抗原
- POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))
- 别名
- POLD1 (POLD1 产品)
- 别名
- CDC2 antibody, CRCS10 antibody, POLD antibody, 5949 antibody, CAA61369 antibody, CG5949 antibody, DNA Pol Delta antibody, Dm Poldelta antibody, Dmel\\CG5949 antibody, Poldelta antibody, dnapol delta antibody, l(3)72Ac antibody, cdc2 antibody, pold antibody, 125kDa antibody, Pol delta antibody, fla antibody, im:7139126 antibody, zgc:152688 antibody, DNA polymerase delta 1, catalytic subunit antibody, DNA-polymerase-delta antibody, polymerase (DNA directed), delta 1, catalytic subunit antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase delta antibody, POLD1 antibody, DNApol-delta antibody, pold1 antibody, LOC100456571 antibody, Pold1 antibody
- 背景
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Background: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3\\\'- to 5\\\'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\\\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196).
Aliases: Polymerase (DNA directed) delta 1 catalytic subunit antibody, CDC2 antibody, CDC2 homolog antibody, CRCS10 antibody, DNA directed DNA polymerase delta 1 antibody, DNA directed polymerase delta 1 antibody, DNA pol delta 1 antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase subunit delta p125 antibody, DPOD1_HUMAN antibody, MDPL antibody, POLD antibody, POLD 1 antibody, POLD1 antibody, Polymerase (DNA directed) delta 1 catalytic subunit 125 kDa antibody, Polymerase (DNA) delta 1 catalytic subunit antibody, Polymerase DNA directed delta 1 catalytic subunit 125kD antibody, polymerase, DNA, delta antibody
- UniProt
- P28340
- 途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA
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