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BAX 抗体 (AA 3-16)

Name: BAX 抗体 (AA 3-16)
SKU: ABIN6252364
Availability: OutOfStock

BAX 适用: 人, 猴 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal SPM336 unconjugated

产品编号 ABIN6252364

发货至: 中国

Datasheet as PDF

抗原 See all BAX 抗体

BAX (BCL2-Associated X Protein (BAX))
抗原表位
37

32

18

17

15

11

10

10

9

8

8

7

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 3-16
适用
253

153

142

46

31

27

25

18

17

10

9

5

3

3

3

2

1

1

1

1

1
人, 猴
宿主
166

94

3

2
小鼠
Compatible Secondaries
克隆类型
180

85
单克隆
标记
137

16

13

12

10

7

5

5

5

5

5

5

5

5

5

5

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This BAX antibody is un-conjugated
应用范围
238

111

72

64

52

52

52

41

38

13

12

8

5

3

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

特异性

Does not react with mouse and rat

纯化方法

Protein G affinity chromatography

免疫原

Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.

克隆位点

SPM336

亚型

IgG1

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应用备注

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

限制

仅限研究用
状态

Liquid

浓度

0.2 mg/mL

缓冲液

PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Aliquot and Store at -20C. Avoid freez-thaw cycles.
抗原

BAX (BCL2-Associated X Protein (BAX))

别名

Bax (BAX 产品)

别名

BAX-ALPHA antibody, bax-A antibody, xBax antibody, xlbax antibody, BAX antibody, bax antibody, fj16e01 antibody, wu:fc50b10 antibody, wu:fj16e01 antibody, BCL2L4 antibody, zgc:112983 antibody, BCL2 associated X, apoptosis regulator antibody, BCL2-associated X protein L homeolog antibody, BCL2-associated X protein antibody, bcl2-associated X protein, a antibody, bcl2-associated X protein, b antibody, BAX antibody, bax.L antibody, bax antibody, baxa antibody, Bax antibody, baxb antibody

背景

Recognizes a protein of 21  kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

基因ID

581

UniProt

Q07812

途径

p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response