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BAX 抗体 (AA 3-16) (CF®405S)

Name: BAX 抗体 (AA 3-16) (CF®405S)
SKU: ABIN6175286
Availability: OutOfStock

BAX 适用: 人, 猴 WB, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal 2D2 CF®405S

产品编号 ABIN6175286

发货至: 中国

Datasheet as PDF

抗原 See all BAX 抗体

BAX (BCL2-Associated X Protein (BAX))
抗原表位
38

32

18

16

15

11

10

10

9

8

8

7

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 3-16
适用
252

154

142

46

31

26

25

18

17

10

9

5

3

3

3

2

1

1

1

1

1
人, 猴
宿主
167

93

3

2
小鼠
Compatible Secondaries
克隆类型
181

84
单克隆
标记
138

16

13

12

10

7

6

6

6

6

6

6

5

5

5

5

5

1

1

1

1

1

1

1

1
This BAX antibody is conjugated to CF®405S
应用范围
237

111

71

65

52

52

51

41

39

13

12

7

5

3

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

原理

Mouse Monoclonal anti-Bax (2D2), CF405S Conjugate

产品特性

Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

免疫原

A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

克隆位点

2D2

亚型

IgG1

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应用备注
Does not react with mouse or rat, others not known
- Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
说明

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.

限制

仅限研究用
状态

Liquid

浓度

100 μg/mL

缓冲液

PBS/0.1 % BSA/0.05 % azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Protect from light
抗原

BAX (BCL2-Associated X Protein (BAX))

别名

Bax (BAX 产品)

别名

BAX-ALPHA antibody, bax-A antibody, xBax antibody, xlbax antibody, BAX antibody, bax antibody, fj16e01 antibody, wu:fc50b10 antibody, wu:fj16e01 antibody, BCL2L4 antibody, zgc:112983 antibody, BCL2 associated X, apoptosis regulator antibody, BCL2-associated X protein L homeolog antibody, BCL2-associated X protein antibody, bcl2-associated X protein, a antibody, bcl2-associated X protein, b antibody, BAX antibody, bax.L antibody, bax antibody, baxa antibody, Bax antibody, baxb antibody

分子量

21 kDa

基因ID

581, 624291

UniProt

Q07812

途径

p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response