Rabbit polyclonal antibody raised against recombinant CASP9.
特异性
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:5000) Immunoprecipitation (1:50-1:200) Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user.
限制
仅限研究用
状态
Liquid
缓冲液
In serum (0.05 % sodium azide)
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store at -20°C. Aliquot to avoid repeated freezing and thawing.