C-JUN 抗体 (pSer63)
Quick Overview for C-JUN 抗体 (pSer63) (ABIN358110)
抗原
See all C-JUN (JUN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer63
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特异性
- This antibody detects AP-1/c-Jun pSer63.
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纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.
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亚型
- Ig Fraction
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anti-Jun Proto-Oncogene (JUN) (AA 31-331) antibody
JUN 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Jun Proto-Oncogene (JUN) (AA 1-70) antibodyJUN 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Jun Proto-Oncogene (JUN) antibodyJUN 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5B1 unconjugated
anti-Jun Proto-Oncogene (JUN) (pThr91) antibodyJUN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Jun Proto-Oncogene (JUN) (AA 1-70) antibodyJUN 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Jun Proto-Oncogene (JUN) (N-Term) antibodyJUN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Jun Proto-Oncogene (JUN) antibodyJUN 适用: 人, 小鼠, 大鼠, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2D11 unconjugated
anti-Jun Proto-Oncogene (JUN) (pSer73) antibodyJUN 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Jun Proto-Oncogene (JUN) antibodyJUN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 9F5 unconjugated
anti-Jun Proto-Oncogene (JUN) antibodyJUN 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Monoclonal 4A11 unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
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别名
- AP-1 / C-Jun
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背景
- The gene for the cJun protein is the putative transforming gene of avian sarcoma virus 17. The protein is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene for this protein is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39
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分子量
- 35676 Da
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基因ID
- 3725, 9606
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UniProt
- P05412
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途径
- MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
抗原
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