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C-JUN 抗体 (pSer73)

Name: C-JUN 抗体 (pSer73)
SKU: ABIN6255488
Availability: OutOfStock

JUN 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Phospho-c-Jun (Ser73) expression in various lysates

ABIN6267309 at 1/200 staining human colon cancer tissue sections by IHC-P.

IHC

IF/ICC

Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

4 images

(1 reference)

产品编号 ABIN6255488

发货至: 中国

Datasheet as PDF

抗原 See all C-JUN (JUN) 抗体

C-JUN (JUN) (Jun Proto-Oncogene (JUN))
抗原表位
41

40

30

29

22

16

16

15

11

11

9

9

8

8

7

7

7

7

7

6

6

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
pSer73
适用
375

204

187

32

28

21

17

17

16

12

11

7

5

4

3

1

1
人, 小鼠, 大鼠, 斑马鱼
宿主
369

14

2
兔
Compatible Secondaries
克隆类型
346

39
多克隆
标记
185

24

16

16

16

15

15

15

14

14

13

7

5

5

5

5

5

2

2

2

2

2
This C-JUN antibody is un-conjugated
应用范围
265

132

120

89

57

44

43

43

39

23

11

8

7

4

3

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-c-Jun (Ser73) Antibody detects endogenous levels of c-Jun only when phosphorylated at Serine 73

交叉反应

人, 小鼠, 大鼠, 斑马鱼

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human c-Jun around the phosphorylation site of Serine 73

亚型

IgG

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应用备注

WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C.Stable for 12 months from date of receipt

有效期

12 months
Wu, Wang, Fang, Huang, Sun, Xiao, Yan: "MFAP5 promotes tumor progression and bone metastasis by regulating ERK/MMP signaling pathways in breast cancer." in: Biochemical and biophysical research communications, Vol. 498, Issue 3, pp. 495-501, (2018) (PubMed).
抗原

C-JUN (JUN) (Jun Proto-Oncogene (JUN))

别名

c-Jun (JUN 产品)

别名

AP-1 antibody, AP1 antibody, c-Jun antibody, fj36h07 antibody, wu:fj36h07 antibody, zgc:65863 antibody, Junc antibody, c-jun antibody, CG2275 antibody, D-Jun antibody, D-jun antibody, D-jun/Jra antibody, DJUN antibody, DJun antibody, Djun antibody, Dmel\\CG2275 antibody, JRA antibody, JUN antibody, Jun antibody, V antibody, cJun antibody, d-JRA antibody, d-Jun antibody, d-jun antibody, dAP-1 antibody, dJRA antibody, dJUN antibody, dJra antibody, dJun antibody, djun antibody, dm-Jun antibody, jra antibody, jun antibody, l(2)46Ef antibody, l(2)IA109 antibody, l(2R)IA109 antibody, Jun proto-oncogene, AP-1 transcription factor subunit antibody, jun proto-oncogene S homeolog antibody, jun proto-oncogene antibody, Jun-related antigen antibody, jun proto-oncogene L homeolog antibody, JUN antibody, Jun antibody, jun.S antibody, jun antibody, Jra antibody, jun.L antibody

背景

Description: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Gene: JUN

分子量

37kDa

基因ID

3725

UniProt

P05412, P17535

途径

MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins