CHEK1 抗体 (pSer317)
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北京 101111
Quick Overview for CHEK1 抗体 (pSer317) (ABIN317988)
抗原
See all CHEK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer317
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特异性
- P-Chk1 (pSer317) antibody detects endogenous levels of p-Chk1 protein.
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纯化方法
- Affinity chromatography
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anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibodyCHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibodyCHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibodyCHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibodyCHEK1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibodyCHEK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibodyCHEK1 适用: 人 WB, IHC (p), DB 宿主: 兔 Polyclonal RB7845-RB18425 unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibodyCHEK1 适用: 小鼠, 小鸡 ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 2G1D5 unconjugated
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应用备注
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ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CHEK1 (Checkpoint Kinase 1 (CHEK1))
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别名
- CHK1
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背景
- Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
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分子量
- approx. 56 kDa
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基因ID
- 1111
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NCBI登录号
- NP_001107593
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UniProt
- O14757
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途径
- p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair
抗原
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