ATG5 抗体 (N-Term) (Alkaline Phosphatase (AP))
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- 抗原 See all ATG5 抗体
- ATG5 (ATG5 Autophagy Related 5 (ATG5))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATG5 antibody is conjugated to Alkaline Phosphatase (AP)
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Predicted molecular weight at ~32.4 kDa. Observed molecular weights at ~48-60 kDa based on ATG12-ATG5 heterodimer.
- 交叉反应
- 人
- 纯化方法
- Peptide Affinity Purified
- 免疫原
- Synthetic peptide from the N-terminal of human ATG5
- Top Product
- Discover our top product ATG5 Primary Antibody
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anti-ATG5 Autophagy Related 5 (ATG5) (AA 101-200) antibody
ATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-275) antibodyATG5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 209-238), (C-Term) antibodyATG5 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7547 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 144-275) antibodyATG5 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 3C7A8 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-119) antibodyATG5 适用: 人 WB, ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal ATG5-2101 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 176-275) antibodyATG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G11 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30) antibodyATG5 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30), (N-Term) antibodyATG5 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (C-Term) antibodyATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 28-275) antibodyATG5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN2868891 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- ATG5 (ATG5 Autophagy Related 5 (ATG5))
- 别名
- ATG5 (ATG5 产品)
- 别名
- APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, CG1643 antibody, DmAtg5 antibody, Dmel\\CG1643 antibody, atg5 antibody, 2010107M05Rik antibody, 3110067M24Rik antibody, AW319544 antibody, Apg5l antibody, Atg5l antibody, C88337 antibody, Paddy antibody, apg5l antibody, zgc:100934 antibody, ATATG5 antibody, AUTOPHAGY 5 antibody, MKP11.20 antibody, MKP11_20 antibody, autophagy related 5 antibody, Autophagy-related 5 antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, autophagy related 5 L homeolog antibody, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antibody, autophagy protein Apg5 family antibody, ATG5 antibody, Atg5 antibody, atg5 antibody, atg5.L antibody, APG5 antibody
- 背景
- ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).
- 基因ID
- 9474
- NCBI登录号
- NP_001273035
- 途径
- Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
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