The antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 472.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
免疫原
ATF-2 (phospho Ser472) antibody was raised against a peptide sequence around phosphorylation site of Serine 472 (E-A-V (p)-A-T) derived from Human ATF-2.
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.