Npc1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Npc1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of Npc1 (mouse).
ELISA: Antibody detection limit dilution 1:16000 Western Blot: Approximately 36 kDa band in lysates of cell lines NIH3T3 and NSO after 1 μg/mL antibody staining.2. The 36 kDa band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.