IL-6 抗体
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- 抗原 See all IL-6 (IL6) 抗体
- IL-6 (IL6) (Interleukin 6 (IL6))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This IL-6 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 特异性
- This antibody detects Interleukin-6.
- 纯化方法
- Affinity chromatography employing immobilized Human IL-6 matrix
- 免疫原
- Highly pure (> 98%) E.coli derived recombinant Human IL-6
- Top Product
- Discover our top product IL6 Primary Antibody
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- 应用备注
- Immunohistochemistry: This antibody stained FFPE sections of human cervical squamous cell carcinoma. Recommended dilution: 2.5 μg/mL (overnight at 4C). Use heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. An HRP-labeledpolymer detection system was used with a DAB chromogen. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (5 ng/mL), aconcentration of 0.05 - 0.08 μg/mL of this antibody is required. Elisa(Indirect): To detect hIL-6 by indirect ELISA (using 100 μL/well antibody solution) a
- 限制
- 仅限研究用
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- 溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
- 有效期
- 12 months
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- 抗原
- IL-6 (IL6) (Interleukin 6 (IL6))
- 别名
- Interleukin-6 (IL6) (IL6 产品)
- 别名
- BSF2 antibody, HGF antibody, HSF antibody, IFNB2 antibody, IL-6 antibody, Il-6 antibody, ILg6 antibody, Ifnb2 antibody, il6 antibody, CHIL-6 antibody, interleukin 6 antibody, interleukin-6 antibody, IL6 antibody, Il6 antibody, il-6 antibody, IL-6 antibody
- 背景
- Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2
- 基因ID
- 3569
- NCBI登录号
- NP_000591
- UniProt
- P05231
- 途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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