IL-6 抗体 (HRP)
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- 抗原 See all IL-6 (IL6) 抗体
- IL-6 (IL6) (Interleukin 6 (IL6))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-6 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- This purified antibody has been heated to 56 °C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
- 纯化方法
- Purified, heated to 56°C for 30 minutes
- 免疫原
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This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E. coli.
Type of Immunogen: Recombinant protein - 亚型
- IgG
- Top Product
- Discover our top product IL6 Primary Antibody
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- 应用备注
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Approved: ELISA, IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)
Usage: This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 23.7 kD in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- avoid freeze thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- 抗原
- IL-6 (IL6) (Interleukin 6 (IL6))
- 别名
- IL6 (IL6 产品)
- 别名
- BSF2 antibody, HGF antibody, HSF antibody, IFNB2 antibody, IL-6 antibody, Il-6 antibody, ILg6 antibody, Ifnb2 antibody, il6 antibody, CHIL-6 antibody, interleukin 6 antibody, interleukin-6 antibody, IL6 antibody, Il6 antibody, il-6 antibody, IL-6 antibody
- 背景
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Name/Gene ID: IL6
Family: Hormone
Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6 - 基因ID
- 3569
- UniProt
- P05231
- 途径
- TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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