FCGR3A Protein (AA 17-208) (His-Avi Tag,Biotin)
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- 抗原 See all FCGR3A 蛋白
- FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-208
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FCGR3A protein is labelled with His-Avi Tag,Biotin.
- 应用范围
- SDS-PAGE (SDS), Surface Plasmon Resonance (SPR), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC)
- 原理
- Biotinylated human Fc gamma RIIIa / CD16a (176V) protein
- 序列
- GMRTEDLPKA VVFLEPQWYR VLEKDSVTLK CQGAYSPEDN STQWFHNESL ISSQASSYFI DAATVDDSGE YRCQTNLSTL SDPVQLEVHI GWLLLQAPRW VFKEEDPIHL RCHSWKNTAL HKVTYLQNGK GRKYFHHNSD FYIPKATLKD SGSYFCRGLV GSKNVSSETV NITITQGLAV STISSFFPPG YQGGGLNDIF EAQKIEWHEG GGENLYFQSG GHHHHHHHHH H
- 特异性
- IgG
- 产品特性
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The sequence of the extracellular domain of human CD16a (Gly 17-Gln 208) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Allotype: 176V - the high affinity polymorphic variant for CD16a. - 纯化方法
- Nickel and SEC
- 纯度
- > 95 % by SEC-HPLC
- 内毒素水平
- <1.0 EU per mg
- Biological Activity Comment
- Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIIa / CD16a (176V) protein, directly immobilized on a CM5 chip, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 235 nM.
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- Discover our top product FCGR3A 蛋白
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Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) protein (His-Avi Tag)
FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) protein (His-Avi Tag,Biotin)FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) protein (His-Avi Tag,Biotin)FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SPR, SEC-HPLC
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) protein (His-Avi Tag)FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SPR, SEC-HPLC
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) protein (His-Avi Tag)FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SPR, SEC-HPLC
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-254) protein (GST tag)FCGR3A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-208) (Active) protein (His tag)FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (AA 17-206) protein (His tag)FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) protein (His tag)FCGR3A 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) (Active) protein (His tag,AVI tag)FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C,-80 °C
- 储存方法
- Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
- 有效期
- 12 months
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- 抗原
- FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
- 别名
- CD16a (FCGR3A 产品)
- 别名
- CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein
- 背景
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CD16A, FCGR3A, FCGRIIIA, FCR3A, FCRIIIA, IGFR3A, IGFRIIIA
Background: Low affinity immunoglobulin gamma Fc receptor IIIa, also known as FcγRIIIa or CD16a, is a type I integral membrane glycoprotein. CD16a is a member of the immunoglobulin superfamily and is expressed on macrophages, monocytes and NK cells. CD16a binds monomeric IgG with low affinity but is efficient at binding immune complexes and functions in NK cell activation, phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). CD16a is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail. CD16a is associated with a dimer of the common Fc receptor gamma-chain which contains the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD16a. CD16a has two allotypic variants differing at amino acid position 176, one containing phenylalanine (F176) and the other valine (V176). The V176 variant has a greater affinity for all the IgG subclasses and thus results in greater effector function. - 分子量
- 26.3 kDa
- UniProt
- P08637
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