The sequence of the extracellular domain of human CD16a (Gly 17-Gln 208) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. Allotype: 176V - the high affinity polymorphic variant for CD16a.
纯化方法
Nickel and SEC
纯度
> 95 % by SEC-HPLC
内毒素水平
<1.0 EU per mg
Biological Activity Comment
Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIIa / CD16a (176V) protein, directly immobilized on a CM5 chip, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 235 nM.
FCGR3A
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Active
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.
浓度
1 mg/mL
缓冲液
PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)
储存液
Without preservative
储存条件
RT,4 °C,-20 °C,-80 °C
储存方法
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
有效期
12 months
抗原
FCGR3A
(Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein
背景
CD16A, FCGR3A, FCGRIIIA, FCR3A, FCRIIIA, IGFR3A, IGFRIIIA Background: Low affinity immunoglobulin gamma Fc receptor IIIa, also known as FcγRIIIa or CD16a, is a type I integral membrane glycoprotein. CD16a is a member of the immunoglobulin superfamily and is expressed on macrophages, monocytes and NK cells. CD16a binds monomeric IgG with low affinity but is efficient at binding immune complexes and functions in NK cell activation, phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). CD16a is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail. CD16a is associated with a dimer of the common Fc receptor gamma-chain which contains the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD16a. CD16a has two allotypic variants differing at amino acid position 176, one containing phenylalanine (F176) and the other valine (V176). The V176 variant has a greater affinity for all the IgG subclasses and thus results in greater effector function.