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FCGR3A Protein (AA 17-208) (His-Avi Tag)

FCGR3A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7274281
发货至: 中国
  • 抗原 See all FCGR3A 蛋白
    FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
    蛋白类型
    Recombinant
    产品特性
    AA 17-208
    宿主
    • 21
    • 3
    • 3
    • 1
    资源
    • 15
    • 6
    • 3
    • 2
    • 1
    HEK-293 Cells
    标记
    This FCGR3A protein is labelled with His-Avi Tag.
    原理
    Human Fc gamma RIIIA/CD16a (F176) Protein
    序列
    Gly17-Gln208 (F176)
    产品特性
    Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 85.6ng/ml determined by ELISA. The affinity constant of 0.92μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
    别名
    Fc gamma RIIIA (FCGR3A 产品)
    别名
    CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein
    背景
    Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
    分子量
    24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
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