NGFB Protein (His tag)
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- 抗原 See all NGFB 蛋白
- NGFB (Nerve Growth Factor beta (NGFB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This NGFB protein is labelled with His tag.
- 原理
- Recombinant Human Beta-NGF/NGF/NGFB Protein
- 序列
- EPHSESNVPA GHTIPQAHWT KLQHSLDTAL RRARSAPAAA IAARVAGQTR NITVDPRLFK KRRLRSPRVL FSTQPPREAA DTQDLDFEVG GAAPFNRTHR SKRSSSHPIF HRGEFSVCDS VSVWVGDKTT ATDIKGKEVM VLGEVNINNS VFKQYFFETK CRDPNPVDSG CRGIDSKHWN SYCTTTHTFV KALTMDGKQA AWRFIRIDTA CVCVLSRKAV RRA
- 特异性
- Glu19-Ala241
- 纯度
- > 92 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- <0.01EU/μg
- Biological Activity Comment
- Recombinant Human NGF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 for this effect is 2.2-8.6 ng/mL, corresponding to a specific activity of 1.16x105-4.55x105 units/mg.
- Top Product
- Discover our top product NGFB 蛋白
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Nerve Growth Factor beta (NGFB) (Active) protein
NGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 浓度
- 0.43 mg/mL
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- NGFB (Nerve Growth Factor beta (NGFB))
- 别名
- Beta-NGF/NGF/NGFB (NGFB 产品)
- 别名
- NGFB Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, hsan5 Protein, beta-ngf Protein, Ngfb Protein, Beta-NGF Protein, HSAN5 Protein, beta-NGF Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, beta-Nerve growth factor Protein, nerve growth factor (beta polypeptide) Protein, NGF Protein, ngfb Protein, FPV072 Protein, FPV076 Protein, ngf Protein, Ngf Protein
- 背景
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Description: NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor?dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Name: NGFB, HSAN5, Beta-NGF
- 基因ID
- 4803
- UniProt
- P01138
- 途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
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