NGFB 蛋白
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北京 101111
Quick Overview for NGFB 蛋白 (ABIN7320297)
抗原
See all NGFB 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Recombinant Mouse β-NGF/Beta-NGF Protein
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序列
- Ser 122-Gly 241
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产品特性
- A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-8ng/mL.
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Nerve Growth Factor beta (NGFB) protein
NGFB 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Nerve Growth Factor beta (NGFB) (AA 130-239) (Active) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. CC Active
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM NaAc, 150 mM NaCl, pH 5.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- NGFB (Nerve Growth Factor beta (NGFB))
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别名
- beta-NGF/Beta-NGF
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背景
- Ngfb,Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
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分子量
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Calculated MW: 13.5 kDa
Observed MW: 13.5 kDa
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基因ID
- 18049
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NCBI登录号
- NP_001106168
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UniProt
- P01139
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途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
抗原
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