CST6 Protein (His tag)
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- 抗原 See all CST6 蛋白
- CST6 (Cystatin E/M (CST6))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CST6 protein is labelled with His tag.
- 原理
- Active Recombinant Human Cystatin-M/CST6 Protein
- 序列
- RPQERMVGEL RDLSPDDPQV QKAAQAAVAS YNMGSNSIYY FRDTHIIKAQ SQLVAGIKYF LTMEMGSTDC RKTRVTGDHV DLTTCPLAAG AQQEKLRCDF EVLVVPWQNS SQLLKHNCVQ M
- 特异性
- Arg29-Met149
- 纯度
- > 95 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.01 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is approximately 16 nM.
- Top Product
- Discover our top product CST6 蛋白
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Cystatin E/M (CST6) (AA 61-149) protein (GST tag)
CST6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cystatin E/M (CST6) (AA 29-149) protein (GST tag)CST6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cystatin E/M (CST6) (AA 29-149) (Active) protein (His tag)CST6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS Active
Cystatin E/M (CST6) protein (Myc-DYKDDDDK Tag)CST6 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- 抗原
- CST6 (Cystatin E/M (CST6))
- 别名
- Cystatin-M/CST6 (CST6 产品)
- 别名
- CST6 Protein, 1110017E11Rik Protein, N28197 Protein, ichq Protein, cystatin E/M Protein, CST6 Protein, Cst6 Protein
- 背景
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Description: Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily. It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning. However, its protein was found only in skin and sweat glands by a third group. In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases. It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age. Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation.
Name: CST6
- 基因ID
- 1474
- UniProt
- Q15828
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