TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This TIGIT protein is labelled with His-Avi Tag,Biotin.
序列
Met22-Pro141
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
TIGIT
宿主: Cynomolgus
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein
背景
TIGIT, VSIG9, VSTM3, WUCAM,TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
分子量
16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.