TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
蛋白类型
Recombinant
产品特性
AA 22-141
宿主
人
资源
HEK-293 Cells
标记
This TIGIT protein is labelled with Fc Tag.
原理
Human TIGIT Protein
序列
Met22-Pro141
产品特性
Recombinant Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met22-Pro141.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD155, mFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 73.0ng/ml determined by ELISA. The affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). See testing image for detail.
TIGIT
宿主: Cynomolgus
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein
背景
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
分子量
39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result.