Sclerostin Protein (SOST) (AA 24-213) (His-Avi Tag)
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- 抗原 See all Sclerostin (SOST) 蛋白
- Sclerostin (SOST)
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-213
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Sclerostin protein is labelled with His-Avi Tag.
- 原理
- Human SOST/Sclerostin Protein
- 序列
- Gln24-Tyr213
- 产品特性
- Recombinant Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human SOST, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Sclerostin (SOST)
- 别名
- SOST (SOST 产品)
- 别名
- LOC100313724 Protein, CDD Protein, VBCH Protein, 5430411E23Rik Protein, sclerostin Protein, LOC100313724 Protein, SOST Protein, Sost Protein
- 背景
- SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
- 分子量
- 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
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