Sclerostin Protein (SOST) (AA 24-213) (Biotin,His-Avi Tag)
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Quick Overview for Sclerostin Protein (SOST) (AA 24-213) (Biotin,His-Avi Tag) (ABIN7275637)
抗原
See all Sclerostin (SOST) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 24-213
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标记
- This Sclerostin protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human SOST/Sclerostin Protein
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序列
- Gln24-Tyr213
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产品特性
- Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.7ng/ml determined by ELISA (QC Test).,Immobilized Anti-SOST Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 9.6ng/ml determined by ELISA.,Biotinylated Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Sclerostin (SOST) (AA 24-213) protein (His-Avi Tag)
SOST 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Sclerostin (SOST) (AA 69-258) (Active) protein (His tag)SOST 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Sclerostin (SOST) protein (His tag)SOST 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
Sclerostin (SOST) protein (His tag)SOST 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE
Sclerostin (SOST) (AA 24-213) protein (His tag,Biotin)SOST 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Sclerostin (SOST)
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别名
- SOST
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背景
- SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
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分子量
- 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9BQB4
抗原
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