PD-1 Protein (AA 25-167) (mFc Tag)
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- 抗原 See all PD-1 (PDCD1) 蛋白
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-167
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This PD-1 protein is labelled with mFc Tag.
- 原理
- Human PD-1/PDCD1 Protein
- 序列
- Leu25-Gln167
- 产品特性
- Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human PD-1, mFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product PDCD1 蛋白
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Programmed Cell Death 1 (PDCD1) (AA 25-167) protein (Fc Tag)
PDCD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Programmed Cell Death 1 (PDCD1) (AA 25-167) protein (His tag)PDCD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Programmed Cell Death 1 (PDCD1) (AA 21-167) protein (Fc Tag)PDCD1 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Programmed Cell Death 1 (PDCD1) protein (Fc-Avi Tag,Biotin)PDCD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Programmed Cell Death 1 (PDCD1) (AA 25-167) protein (His-Avi Tag,Biotin)PDCD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Programmed Cell Death 1 (PDCD1) protein (Fc-His Tag)PDCD1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Programmed Cell Death 1 (PDCD1) (AA 25-167) protein (Fc Tag)PDCD1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Programmed Cell Death 1 (PDCD1) (AA 25-167) protein (Fc Tag,Biotin)PDCD1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Programmed Cell Death 1 (PDCD1) protein (mFc-His Tag)PDCD1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Programmed Cell Death 1 (PDCD1) (AA 21-169) protein (His tag)Crystallography grade PDCD1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
- 别名
- PD-1 (PDCD1 产品)
- 别名
- CD279 Protein, PD-1 Protein, PD1 Protein, SLEB2 Protein, hPD-1 Protein, hPD-l Protein, Ly101 Protein, Pdc1 Protein, programmed cell death 1 Protein, PDCD1 Protein, Pdcd1 Protein
- 背景
- Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.
- 分子量
- 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Tris-Bis PAGE result.
- 途径
- Cancer Immune Checkpoints
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