MICA
(MHC Class I Polypeptide-Related Sequence A (MICA))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This MICA protein is labelled with His-Avi Tag,Biotin.
序列
Glu24-Gln308
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32μg/ml determined by ELISA. See testing image for detail.
MICA
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
MICA
(MHC Class I Polypeptide-Related Sequence A (MICA))
MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein
背景
FLJ60820, MGC111087, MICA, PERB11.1, DAMA-345G11.2, FLJ36918, FLJ60820, MGC21250,TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
分子量
35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.