KREMEN2
(Kringle Containing Transmembrane Protein 2 (KREMEN2))
蛋白类型
Recombinant
产品特性
AA 26-364
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This KREMEN2 protein is labelled with His tag.
原理
Cynomolgus Kremen-2 Protein
序列
Gly26-Ala364
产品特性
Recombinant Cynomolgus Kremen-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly26-Ala364.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus Kremen-2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Kremen-2 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
KREMEN2
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by reduced SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
KREMEN2
(Kringle Containing Transmembrane Protein 2 (KREMEN2))
krm2 Protein, KREMEN2 Protein, MGC147633 Protein, KRM2 Protein, 2900054E04Rik Protein, Krm2 Protein, kringle containing transmembrane protein 2 S homeolog Protein, kringle containing transmembrane protein 2 Protein, kremen2.S Protein, KREMEN2 Protein, kremen2 Protein, Kremen2 Protein
背景
Kremen2 (Krm2) plays an important role in embryonic development, bone formation, and tumorigenesis as a crucial regulator of classical Wnt/β-catenin signaling pathway. Compared to para-cancerous tissues, Krm2 was significantly up-regulated in gastric cancer tissues and was positively correlated with the pathological grade of gastric cancer patients. Krm2 can be a potent candidate for designing of targeted therapy.
分子量
37.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.