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Glypican 3 Protein (GPC3) (AA 25-559) (His tag)

GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7274774
发货至: 中国
  • 抗原 See all Glypican 3 (GPC3) 蛋白
    Glypican 3 (GPC3)
    蛋白类型
    Recombinant
    产品特性
    AA 25-559
    宿主
    • 23
    • 6
    • 6
    • 4
    • 1
    资源
    • 26
    • 7
    • 3
    • 2
    • 1
    • 1
    HEK-293 Cells
    标记
    This Glypican 3 protein is labelled with His tag.
    原理
    Human GPC3/Glypican 3 Protein
    序列
    Gln25-His559
    产品特性
    Recombinant Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    Glypican 3 (GPC3)
    别名
    GPC3 (GPC3 产品)
    别名
    GPC3 Protein, sgb Protein, dgsx Protein, sdys Protein, sgbs Protein, oci-5 Protein, sgbs1 Protein, DGSX Protein, GTR2-2 Protein, MXR7 Protein, OCI-5 Protein, SDYS Protein, SGB Protein, SGBS Protein, SGBS1 Protein, Glypican-3 Protein, glypican 3 Protein, gpc3 Protein, GPC3 Protein, Gpc3 Protein
    背景
    Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
    分子量
    61.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result.
    途径
    Glycosaminoglycan Metabolic Process
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