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Glypican 3 Protein (GPC3) (AA 25-559) (His tag,AVI tag,Biotin)

This Recombinant Glypican 3 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.
产品编号 ABIN5954920
发货至: 中国
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Quick Overview for Glypican 3 Protein (GPC3) (AA 25-559) (His tag,AVI tag,Biotin) (ABIN5954920)

抗原

See all Glypican 3 (GPC3) 蛋白
Glypican 3 (GPC3)

蛋白类型

Recombinant

生物活性

Active

宿主

  • 24
  • 12
  • 9
  • 3
  • 1

资源

  • 28
  • 8
  • 3
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  • 2
  • 1
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  • 1
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  • 1
HEK-293 Cells

纯度

>85 % as determined by SDS-PAGE.
  • 产品特性

    AA 25-559

    标记

    This Glypican 3 protein is labelled with His tag,AVI tag,Biotin.

    序列

    AA 25-559

    特异性

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

    内毒素水平

    Less than 1.0 EU per μg by the LAL method.
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  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制

    仅限研究用
  • 状态

    Lyophilized

    缓冲液

    PBS, pH 7.4

    注意事项

    Please avoid repeated freeze-thaw cycles.

    储存条件

    -20 °C
  • Berman, Kelada, Gutsche, Natarajan, Swenson, Fu, Hong, Ho, Choyke, Escorcia: "In Vitro Performance of Published Glypican 3-Targeting Peptides TJ12P1 and L5 Indicates Lack of Specificity and Potency." in: Cancer biotherapy & radiopharmaceuticals, (2019) (PubMed).

  • 抗原

    Glypican 3 (GPC3)

    别名

    Glypican 3

    背景

    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

    分子量

    38.1 kDa and 26.4 kDa

    NCBI登录号

    NP_004475

    途径

    Glycosaminoglycan Metabolic Process
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