Glypican 3 Protein (GPC3) (AA 25-559) (Biotin,His-Avi Tag)
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Quick Overview for Glypican 3 Protein (GPC3) (AA 25-559) (Biotin,His-Avi Tag) (ABIN7274775)
抗原
See all Glypican 3 (GPC3) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 25-559
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标记
- This Glypican 3 protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human GPC3/Glypican 3 Protein
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序列
- Gln25-His559
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产品特性
- Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln25-His559.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human GPC3, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test).,Biotinylated Human GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Use Biotinylated Human GPC3-His protein to detect the expression rate of Anti-GPC3-CAR positive cells. 293T cells and anti-GPC3 CAR-293T cells were incubated with Biotinylated human GPC3-His (25-559) , His Tag and Biotinylated Protein Control-His. Non-transfected 293 cells and Biotinylated Protein Control-His were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human GPC3.,The purity of Biotinylated Human GPC3 is greater than 95% as determined by SEC-HPLC.
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Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag)
GPC3 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Glypican 3 (GPC3) (AA 99-330) protein (Fc Tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Glypican 3 (GPC3) (AA 438-554) (Active) protein-VLPGPC3 宿主: 人 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC ELISA, Imm, Func, SPR Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag,AVI tag,Biotin)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) protein (His tag)GPC3 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Glypican 3 (GPC3) protein (His tag)GPC3 宿主: 猕猴 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Glypican 3 (GPC3) (AA 301-400) protein (GST tag)GPC3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the Certificate of Analysis for detailed instructions.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Glypican 3 (GPC3)
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别名
- GPC3
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背景
- Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
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分子量
- 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 68-80 kDa based on Bis-Tris PAGE result.
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UniProt
- P51654
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途径
- Glycosaminoglycan Metabolic Process
抗原
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