This LI Cadherin protein is labelled with His tag.
原理
Human CDH17/Cadherin 17 Domain 1 & 2 Protein
序列
Pro30-Pro244
产品特性
Recombinant Human CDH17/Cadherin 17 Domain 1 & 2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro30-Pro244.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CDH17 Domain 1&2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CDH17 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Liver-intestine cadherin (CDH17) has been known to function as a tumor stimulator and diagnostic marker for almost two decades.In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.
分子量
25.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.