This 2B4 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human 2B4/CD244/SLAMF4 Protein
序列
Cys22-Arg221
产品特性
Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human 2B4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD48, hFc Tag with the Ec50 of 7.2ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
CD244
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
分子量
24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Tris-Bis PAGE result.