2B4 Protein (AA 22-221) (His tag,AVI tag,Biotin)
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- 抗原 See all 2B4 (CD244) 蛋白
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-221
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This 2B4 protein is labelled with His tag,AVI tag,Biotin.
- 品牌
- PrecisionAvi
- 序列
- AA 22-221
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.4 kDa. The protein migrates as 40-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product CD244 蛋白
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- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
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- 抗原
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 别名
- 2B4 (CD244 产品)
- 别名
- CD244 Protein, 2B4 Protein, NAIL Protein, NKR2B4 Protein, Nmrk Protein, SLAMF4 Protein, C9.1 Protein, F730046O15Rik Protein, Ly90 Protein, CD244 molecule Protein, CD244 natural killer cell receptor 2B4 Protein, CD244 Protein, Cd244 Protein
- 背景
- Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
- 分子量
- 25.4 kDa
- NCBI登录号
- NP_057466
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