2B4 Protein (AA 22-221) (His tag)
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北京 101111
Quick Overview for 2B4 Protein (AA 22-221) (His tag) (ABIN7273780)
抗原
See all 2B4 (CD244) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 22-221
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标记
- This 2B4 protein is labelled with His tag.
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原理
- Human 2B4/CD244/SLAMF4 Protein
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序列
- Cys22-Arg221
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产品特性
- Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Arg221.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human 2B4, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA.,Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Human 2B4, His Tag with an affinity constant of 0.51 μM as determined in BLI assay (Gator® Prime).,The purity of Human 2B4 is greater than 90% as determined by SEC-HPLC.
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Natural Killer Cell Receptor 2B4 (CD244) (AA 22-221) (Active) protein (Biotin,His-Avi Tag)
CD244 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Natural Killer Cell Receptor 2B4 (CD244) (AA 22-216) protein (His tag,mFc Tag)CD244 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Natural Killer Cell Receptor 2B4 (CD244) (AA 22-216) protein (Fc Tag)CD244 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Natural Killer Cell Receptor 2B4 (CD244) (AA 22-221) protein (His tag,AVI tag,Biotin)CD244 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Natural Killer Cell Receptor 2B4 (CD244) protein (His tag)CD244 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Natural Killer Cell Receptor 2B4 (CD244) (AA 20-225) protein (Fc Tag)CD244 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Natural Killer Cell Receptor 2B4 (CD244) (AA 1-365) protein (GST tag)CD244 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Natural Killer Cell Receptor 2B4 (CD244) (AA 19-224) protein (His tag)CD244 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
Natural Killer Cell Receptor 2B4 (CD244) (AA 19-224) (Active) protein (hIgG-His-tag)CD244 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS, AcA Active
Natural Killer Cell Receptor 2B4 (CD244) (AA 22-221) (Active) protein (Fc Tag)CD244 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 24 months
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- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
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别名
- 2B4
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背景
- 2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
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分子量
- 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9BZW8
抗原
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