Sonic Hedgehog Protein (SHH) (His tag)
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- 抗原 See all Sonic Hedgehog (SHH) 蛋白
- Sonic Hedgehog (SHH)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This Sonic Hedgehog protein is labelled with His tag.
- 原理
- Recombinant Mouse Sonic Hedgehog/SHH Protein (His Tag)(Active)
- 序列
- Met1-Gly198
- 产品特性
- A DNA sequence encoding the mouse SHH (Q62226) (Met1-Gly198) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 90 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465.The ED50 for this effect is typically 5-40 μg/mL.
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- Discover our top product SHH 蛋白
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Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197) proteinSHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 48-197) protein (His-IF2DI Tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Sonic Hedgehog (SHH) protein (His tag)SHH 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) proteinSHH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Sonic Hedgehog (SHH) (AA 198-462) protein (His tag)Crystallography grade SHH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Sonic Hedgehog (SHH)
- 别名
- SHH (SHH 产品)
- 别名
- HHG1 Protein, HLP3 Protein, HPE3 Protein, MCOPCB5 Protein, SMMCI Protein, TPT Protein, TPTPS Protein, 9530036O11Rik Protein, Dsh Protein, Hhg1 Protein, Hx Protein, Hxl3 Protein, M100081 Protein, fc83d08 Protein, shh Protein, syu Protein, vhh-1 Protein, vhh1 Protein, wu:fc83d08 Protein, Xhh Protein, hedgehog Protein, xshh Protein, SHH Protein, twh Protein, twhh Protein, sonic hedgehog Protein, sonic hedgehog a Protein, sonic hedgehog L homeolog Protein, sonic hedgehog protein A Protein, sonic hedgehog b Protein, SHH Protein, Shh Protein, shha Protein, shh.L Protein, shh Protein, shhb Protein
- 背景
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Background: Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine, liver, lung, and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.
Synonym: Sonic Hedgehog Protein, SHH, HHG-1, SHH,9530036O11Rik, Dsh, Hhg1, Hx, Hxl3, M10008
- 分子量
- 21 kDa
- UniProt
- Q62226
- 途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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