KIT Protein (His tag)
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- 抗原 See all KIT 蛋白
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This KIT protein is labelled with His tag.
- 原理
- Recombinant Mouse c-kit/CD117 Protein (His Tag)(Active)
- 序列
- Met 1-Thr 523
- 产品特性
- A DNA sequence encoding the mouse KIT isoform 1 (NP_001116205.1) extracellular domain (Met 1-Thr 523) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 97 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized mouse KIT at 2 μg/ml (100 μl/well) can bind biotinylated mouse KITL with a linear ranger of 1.28-6.4 ng/ml.
- Top Product
- Discover our top product KIT 蛋白
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Mast/stem Cell Growth Factor Receptor (KIT) protein (His tag)
KIT 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Mast/stem Cell Growth Factor Receptor (KIT) (Val560Gly-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Thr670Ile-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 544-976) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Asp816Tyr-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Asp816Val-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Thr670Ile-Mutant) (Active) protein (His-GST)KIT 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 544-976) (Active) protein (His-GST)KIT 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-516) (Active) protein (Fc Tag)KIT 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 1-976) protein (GST tag)KIT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- 别名
- c-kit/CD117 (KIT 产品)
- 别名
- C-Kit Protein, CD117 Protein, PBT Protein, SCFR Protein, Bs Protein, Fdc Protein, Gsfsco1 Protein, Gsfsco5 Protein, Gsfsow3 Protein, SCO1 Protein, SCO5 Protein, SOW3 Protein, Ssm Protein, Tr-kit Protein, W Protein, c-KIT Protein, c-kit Protein, MGF Protein, KIT Protein, C-KIT Protein, kit Protein, receptor Protein, sparse Protein, Xkl-1 Protein, kit-a Protein, kit-b Protein, kl1-A Protein, krk1 Protein, pbt Protein, scfr Protein, KIT proto-oncogene receptor tyrosine kinase Protein, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a Protein, KIT proto-oncogene receptor tyrosine kinase S homeolog Protein, KIT Protein, Kit Protein, kita Protein, kit Protein, kit.S Protein
- 背景
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Background: C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: Bs;c-KIT;CD117;Fdc;Gsfsco1;Gsfsco5;Gsfsow3;SCO1;SCO5;SOW3;Ssm;Tr-kit;W
- 分子量
- 57 kDa
- NCBI登录号
- NP_001116205
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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