CDK4 Protein (GST tag)
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- 抗原 See all CDK4 蛋白
- CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This CDK4 protein is labelled with GST tag.
- 原理
- Recombinant Human CDK4 Protein (GST Tag)
- 序列
- Met 1-Glu 303
- 产品特性
- A DNA sequence encoding the human CDK4 (NP_000066.1) (Met 1-Glu 303) was fused with the GST tag at the N-terminus.
- 纯度
- > 80 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product CDK4 蛋白
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Cyclin-Dependent Kinase 4 (CDK4) (AA 1-303) protein (GST tag)
CDK4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 4 (CDK4) (AA 1-303) (Active) protein (GST tag)CDK4 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 4 (CDK4) (AA 4-303) (Active) protein (His tag)CDK4 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 4 (CDK4) (AA 4-303) (Active) protein (GST tag)CDK4 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 4 (CDK4) (AA 207-303) protein (His-IF2DI Tag)CDK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin-Dependent Kinase 4 (CDK4) (AA 1-303) protein (His tag)CDK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cyclin-Dependent Kinase 4 (CDK4) (AA 211-303) protein (GST tag)CDK4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 4 (CDK4) (AA 2-303) protein (His tag)Crystallography grade CDK4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 10 % glycerol, 0.5 mM PMSF, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
- 别名
- CDK4 (CDK4 产品)
- 别名
- zgc:153726 Protein, CMM3 Protein, PSK-J3 Protein, Crk3 Protein, 8-6 Protein, CDK4 Protein, CDK4/6 Protein, CG5072 Protein, Cdk4/6 Protein, DmCdk4 Protein, Dmel\\CG5072 Protein, Pk53C Protein, Pk?7 Protein, cdk Protein, cdk4 Protein, cdk4/6 Protein, dCdk4 Protein, l(2)05428 Protein, l(2)0671 Protein, l(2)k06503 Protein, l(2)s4639 Protein, l(2)sh0671 Protein, xcdk4 Protein, cyclin-dependent kinase 4 Protein, cyclin dependent kinase 4 Protein, Cyclin-dependent kinase 4 Protein, cyclin-dependent kinase 4 L homeolog Protein, cdk4 Protein, CDK4 Protein, Cdk4 Protein, cdk4.L Protein
- 背景
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Background: CDK4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). CDK4 was shown to be responsible for the phosphorylation of retinoblastoma gene product. CDK4 is the ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. CDK4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma.
Synonym: CMM3,PSK-J3
- 分子量
- 60 kDa
- NCBI登录号
- NP_000066
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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