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SNCA Protein (Ala53Thr-Mutant)

SNCA 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant SDS, WB, in vitro, in vivo Active
产品编号 ABIN6952303
发货至: 中国
  • 抗原 See all SNCA 蛋白
    SNCA (Synuclein, alpha (SNCA))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    Ala53Thr-Mutant
    宿主
    • 24
    • 7
    • 4
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    • 1
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    资源
    • 25
    • 13
    • 3
    • 2
    大肠杆菌(E. Coli)
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)
    序列
    MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
    特异性
    ~14.46 kDa
    产品特性
    Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1)
    纯化方法
    Ion-exchange Purified
    Biological Activity Comment
    100 μM A53T alpha synuclein protein monomer (SPR-325) seeded with 10 nM A53T alpha synuclein protein PFF (SPR-326) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
  • 说明

    Certified >95% pure using SDS-PAGE analysis.

    限制
    仅限研究用
  • 浓度
    Lot specific
    缓冲液
    PBS pH 7.4
    储存条件
    -80 °C
  • 抗原
    SNCA (Synuclein, alpha (SNCA))
    别名
    Alpha Synuclein (SNCA 产品)
    别名
    snca Protein, MGC64356 Protein, LOC619283 Protein, NACP Protein, alphaSYN Protein, PARK1 Protein, PARK4 Protein, PD1 Protein, synuclein alpha L homeolog Protein, alpha-synuclein Protein, synuclein alpha Protein, synuclein, alpha Protein, snca.L Protein, LOC619283 Protein, SNCA Protein, Snca Protein
    背景
    Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus. Functionally, it has been shown to significantly interact with tubulin, and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory. SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease and increased rates of alpha synuclein fibrillization.
    基因ID
    6622
    NCBI登录号
    NP_000336
    途径
    Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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