NFAT1 Protein (AA 396-678) (His tag)
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- 抗原 See all NFAT1 蛋白
- NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 396-678
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This NFAT1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- PLEWPLSSQS GSYELRIEVQ PKPHHRAHYE TEGSRGAVKA PTGGHPVVQL HGYMENKPLG LQIFIGTADE RILKPHAFYQ VHRITGKTVT TTSYEKIVGN TKVLEIPLEP KNNMRATIDC AGILKLRNAD IELRKGETDI GRKNTRVRLV FRVHIPESSG RIVSLQTASN PIECSQRSAH ELPMVERQDT DSCLVYGGQQ MILTGQNFTS ESKVVFTEKT TDGQQIWEME ATVDKDKSQP NMLFVEIPEY RNKHIRTPVK VNFYVINGKR KRSQPQHFTY HPVHHHHHH
- 纯度
- > 90 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product NFAT1 蛋白
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Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (AA 812-921) protein (GST tag)
NFAT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (AA 1-925) protein (Strep Tag)Crystallography grade NFAT1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (AA 1-927) protein (Strep Tag)Crystallography grade NFAT1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NFAT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NFAT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (Transcript Variant D) protein (Myc-DYKDDDDK Tag)NFAT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 40 % glycerol, 1 mM DTT.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))
- 别名
- NFATC2 (NFAT1 产品)
- 别名
- NFATC2 Protein, AI607462 Protein, NF-ATc2 Protein, NFAT1 Protein, NFAT1-D Protein, Nfatp Protein, NFATP Protein, nuclear factor of activated T-cells 2 Protein, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 Protein, NFATC2 Protein, nfatc2 Protein, Nfatc2 Protein
- 背景
- NFATC2, also known as nuclear factor of activated T-cells cytoplasmic 2 isoform C, is a member of the nuclear factor of activated T cells family. This protein plays a vital role in the course of T helper cell activation, differentiation, and effector function. Although KO of an individual NFAT isoform in T cells leads to rather mild effects, T cells deficient for NFATC1 and 2 completely fail to produce T helper cell effector cytokines, such as the interleukins IL-2 and IL-4 or interferon gamma. Also, it is highly phosphorylated and retained in the cytoplasm. Following T cell receptor (TCR) stimulation, dephosphorylation by calcium-activated calcineurin induces a conformational change of NFATC2 that exposes one or more nuclear localization sequences. Recombinant human NFATC2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 33.1kDa (290aa) 28-40kDa (SDS-PAGE under reducing conditions.)
- NCBI登录号
- NP_775114
- UniProt
- Q13469
- 途径
- RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling
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