NAMPT Protein (AA 1-491) (DYKDDDDK Tag)
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- 抗原 See all NAMPT 蛋白
- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-491
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This NAMPT protein is labelled with DYKDDDDK Tag.
- 原理
- Nampt (mouse) (rec.) (enzymatically active)
- 特异性
- Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.
- 产品特性
- Protein. Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/μg purified protein (LAL test, Lonza). Liquid. Contains PBS. Purity: >90 % (SDS-PAGE). Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column.
- 纯度
- >90 % (SDS-PAGE)
- 内毒素水平
- <0.01EU/μg purified protein (LAL test, Lonza).
- Biological Activity Comment
- 0.5μg to 5μg of Nampt (mouse) (rec.) (enzymatically active) show activity in a colorimetric Nampt assay.
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- Discover our top product NAMPT 蛋白
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Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) (Active) protein (His tag)
NAMPT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % (SDS-PAGE) SDS Active
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-368) protein (GST tag)NAMPT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 27-491) protein (His tag)NAMPT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) protein (Strep Tag)Crystallography grade NAMPT 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) protein (Strep Tag)Crystallography grade NAMPT 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. Contains PBS.
- 注意事项
- After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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- 抗原
- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
- 别名
- Nampt (NAMPT 产品)
- 别名
- 1110035O14Rik Protein, PBEF Protein, PBEF1 Protein, VF Protein, VISFATIN Protein, AI314458 Protein, AI480535 Protein, NAmPRTase Protein, Pbef Protein, Pbef1 Protein, Visfatin Protein, visfatin Protein, pbef Protein, pbef1 Protein, nicotinamide phosphoribosyltransferase Protein, Nicotinamide phosphoribosyltransferase Protein, nicotinamide phosphoribosyltransferase S homeolog Protein, NAMPT Protein, Nampt Protein, nampt Protein, Smon_0680 Protein, Mrub_2845 Protein, Cseg_3704 Protein, BC1002_3997 Protein, Ftrac_2686 Protein, Varpa_5681 Protein, Celal_3900 Protein, Deima_0880 Protein, BC1001_3724 Protein, Celly_1696 Protein, Clole_1542 Protein, Tsp_05934 Protein, nampt.S Protein
- 背景
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Alternate Names/Synonyms: Visfatin, PBEF, Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase, NAmPRTase
Product Description: Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column.
- UniProt
- Q99KQ4
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