NAMPT Protein (AA 1-491) (His tag)
(2 references)-
- 抗原 See all NAMPT 蛋白
- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-491
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NAMPT protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 交叉反应
- 人
- 产品特性
- Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
- 纯度
- >90 % (SDS-PAGE)
- 内毒素水平
- <1EU/μg purified protein (LAL test, Lonza).
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Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-368) protein (GST tag)
NAMPT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 27-491) protein (His tag)NAMPT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) protein (Strep Tag)Crystallography grade NAMPT 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) protein (Strep Tag)Crystallography grade NAMPT 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute in distilled water.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized. Salt-free.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C. - 有效期
- 3 months
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: "Expression and regulation of nampt in human islets." in: PLoS ONE, Vol. 8, Issue 3, pp. e58767, (2013) (PubMed).
: "Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway." in: FEBS letters, Vol. 580, Issue 28-29, pp. 6635-43, (2006) (PubMed).
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: "Expression and regulation of nampt in human islets." in: PLoS ONE, Vol. 8, Issue 3, pp. e58767, (2013) (PubMed).
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- 抗原
- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
- 别名
- Nampt (Visfatin/PBEF) (NAMPT 产品)
- 别名
- 1110035O14Rik Protein, PBEF Protein, PBEF1 Protein, VF Protein, VISFATIN Protein, AI314458 Protein, AI480535 Protein, NAmPRTase Protein, Pbef Protein, Pbef1 Protein, Visfatin Protein, visfatin Protein, pbef Protein, pbef1 Protein, nicotinamide phosphoribosyltransferase Protein, Nicotinamide phosphoribosyltransferase Protein, nicotinamide phosphoribosyltransferase S homeolog Protein, NAMPT Protein, Nampt Protein, nampt Protein, Smon_0680 Protein, Mrub_2845 Protein, Cseg_3704 Protein, BC1002_3997 Protein, Ftrac_2686 Protein, Varpa_5681 Protein, Celal_3900 Protein, Deima_0880 Protein, BC1001_3724 Protein, Celly_1696 Protein, Clole_1542 Protein, Tsp_05934 Protein, nampt.S Protein
- 背景
- Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
- 分子量
- ~52kDa (SDS-PAGE)
- UniProt
- P43490
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