GAS6 Protein (AA 136-311) (His tag)
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- 抗原 See all GAS6 蛋白
- GAS6 (Growth Arrest-Specific 6 (GAS6))
- 生物活性
- Active
- 产品特性
- AA 136-311
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GAS6 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- Growth arrest-specific 6, also known as GAS6, is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation. It has been reported that both PC-3 and DU 145 human prostate cancer cell lines are stimulated to proliferate by Gas6, however, this proliferative response strictly correlates with the expression of the Axl receptor, being higher in DU 145 cells. To test the proliferative effect of Gas6, DU 145 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of GAS6. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. Cell proliferation of DU145 cells after incubation with GAS6 for 72h observed by inverted microscope was shown in Figure 1.
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Growth Arrest-Specific 6 (GAS6) (AA 31-637) protein (His tag)
GAS6 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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Growth Arrest-Specific 6 (GAS6) (AA 28-674) protein (His tag)Crystallography grade GAS6 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 5.6
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- GAS6 (Growth Arrest-Specific 6 (GAS6))
- 别名
- Growth Arrest Specific Protein 6 (GAS6) (GAS6 产品)
- 别名
- fj34a12 Protein, zgc:63860 Protein, wu:fj34a12 Protein, MGC68463 Protein, gas6 Protein, MGC83872 Protein, GAS6 Protein, Gas-6 Protein, AXLLG Protein, AXSF Protein, growth arrest specific 6 Protein, growth arrest-specific 6 Protein, growth arrest specific 6 L homeolog Protein, growth arrest specific 6 S homeolog Protein, GAS6 Protein, gas6 Protein, gas6.L Protein, gas6.S Protein, Gas6 Protein
- 背景
- Alternative Names: AXLLG, AXSF, AXL Stimulatory Factor, AXL receptor tyrosine kinase ligand
- 分子量
- 24kDa
- UniProt
- Q14393
- 途径
- RTK signaling, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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