GAS6 Protein (AA 31-721) (His tag)
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- 抗原 See all GAS6 蛋白
- GAS6 (Growth Arrest-Specific 6 (GAS6))
- 蛋白类型
- Recombinant
- 产品特性
- AA 31-721
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宿主
- 人
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资源
- Insect Cells
- 标记
- This GAS6 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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ALLPAREATQ FLRPRQRRAF QVFEEAKQGH LERECVEELC SREEAREVFE NDPETDYFYP RYLDCINKYG SPYTKNSGFA TCVQNLPDQC TPNPCDRKGT QACQDLMGNF FCLCKAGWGG RLCDKDVNEC SQENGGCLQI CHNKPGSFHC SCHSGFELSS DGRTCQDIDE CADSEACGEA RCKNLPGSYS CLCDEGFAYS SQEKACRDVD ECLQGRCEQV CVNSPGSYTC HCDGRGGLKL SQDMDTCELE AGWPCPRHRR DGSPAARPGR GAQGSRSEGH IPDRRGPRPW QDILPCVPFS VAKSVKSLYL GRMFSGTPVI RLRFKRLQPT RLVAEFDFRT FDPEGILLFA GGHQDSTWIV LALRAGRLEL QLRYNGVGRV TSSGPVINHG MWQTISVEEL ARNLVIKVNR DAVMKIAVAG DLFQPERGLY HLNLTVGGIP FHEKDLVQPI NPRLDGCMRS WNWLNGEDTT IQETVKVNTR MQCFSVTERG SFYPGSGFAF YSLDYMRTPL DVGTESTWEV EVVAHIRPAA DTGVLFALWA PDLRAVPLSV ALVDYHSTKK LKKQLVVLAV EHTALALMEI KVCDGQEHVV TVSLRDGEAT LEVDGTRGQS EVSAAQLQER LAVLERHLRS PVLTFAGGLP DVPVTSAPVT AFYRGCMTLE VNRRLLDLDE AAYKHSDITA HSCPPVEPAA A
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GAS6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product GAS6 蛋白
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Growth Arrest-Specific 6 (GAS6) (AA 31-637) protein (His tag)
GAS6 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Growth Arrest-Specific 6 (GAS6) (AA 49-678) protein (His tag)GAS6 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Growth Arrest-Specific 6 (GAS6) (Active) protein (His tag)GAS6 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Growth Arrest-Specific 6 (GAS6) (AA 1-678) protein (GST tag)GAS6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Growth Arrest-Specific 6 (GAS6) (AA 579-678) protein (GST tag)GAS6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Growth Arrest-Specific 6 (GAS6) (AA 136-311) (Active) protein (His tag)GAS6 宿主: 人 宿主: 大肠杆菌（E. Coli） > 90 % AcA, CC Active
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- GAS6 (Growth Arrest-Specific 6 (GAS6))
- 别名
- GAS6 (GAS6 产品)
- 别名
- fj34a12 Protein, zgc:63860 Protein, wu:fj34a12 Protein, MGC68463 Protein, gas6 Protein, MGC83872 Protein, GAS6 Protein, Gas-6 Protein, AXLLG Protein, AXSF Protein, growth arrest specific 6 Protein, growth arrest-specific 6 Protein, growth arrest specific 6 L homeolog Protein, growth arrest specific 6 S homeolog Protein, GAS6 Protein, gas6 Protein, gas6.L Protein, gas6.S Protein, Gas6 Protein
- 背景
- Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. {ECO:0000269|PubMed:12364394, ECO:0000269|PubMed:18840707}., (Microbial infection) Can bridges virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry (PubMed:21501828). Plays a role in Dengue cell entry by apoptotic mimicry (PubMed:23084921). Plays a role in Vaccinia virus cell entry by apoptotic mimicry (PubMed:21501828). Plays a role in ebolavirus and marburgvirus cell entry by apoptotic mimicry (PubMed:17005688). {ECO:0000269|PubMed:17005688, ECO:0000269|PubMed:21501828, ECO:0000269|PubMed:23084921}.
- 分子量
- 77.6 kDa Including tag.
- UniProt
- Q14393
- 途径
- RTK signaling, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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