The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.Suspend BclXL in 100ul of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system. Bcl-XL has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Bcl-XL Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing AA 1-210. The Bcl-XL is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques. Introduction: Bcl-XL is a transmembrane protein located in the mitochondrial membranes of cells that are long-lived and postmitotic, such as adult brain cells. It plays arole in the signal transduction pathway of the FAS-Ligand. Bcl-XL is an anti-apoptotic protein which is a member of the Bcl-2 family which are able to form heterodimers, and this is an significant event in the regulation of apoptosis. BCL-XL is involved in the survival of cancer cells. Bcl-xL is the leading monitor of apoptosis/active cell suicide. Bcl-xL has cell death repressor activity and therefore acts as a survival protein. Synonyms: BclXL, Bcl-X(L), Bcl-XL.