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- 抗原 See all AKT3 蛋白
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 应用范围
- Western Blotting (WB)
- 纯化方法
- Recombinant protein corresponds to amino acids 1 to 481 of mature human AKT3, Akt isoform 3. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
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V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 106-479) (Active) protein (His-GST)
AKT3 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 1-479) protein (GST tag)AKT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 100-189) protein (GST tag)AKT3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 106-479) protein (GST tag,His tag)AKT3 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (Active) proteinAKT3 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE Active
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 2-479) protein (His tag)Crystallography grade AKT3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 2-479) protein (His tag)Crystallography grade AKT3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)AKT3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- 应用备注
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Application Note: AKT3 is suitable as a control for polyclonal or monoclonal anti-AKT3 in immunological assays. Akt3 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.03 mg/mL
- 缓冲液
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Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol
Stabilizer: 10 % (v/v) Glycerol
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 12 months
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- 抗原
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- 别名
- AKT3 Protein (AKT3 产品)
- 别名
- MPPH Protein, PKB-GAMMA Protein, PKBG Protein, PRKBG Protein, RAC-PK-gamma Protein, RAC-gamma Protein, STK-2 Protein, AI851531 Protein, D930002M15Rik Protein, Nmf350 Protein, Pkbg Protein, AKT serine/threonine kinase 3 Protein, thymoma viral proto-oncogene 3 Protein, v-akt murine thymoma viral oncogene homolog 3b Protein, AKT3 Protein, Akt3 Protein, akt3b Protein
- 基因ID
- 10000
- UniProt
- Q9Y243
- 途径
- PI3K-Akt Signaling, RTK signaling, TLR signaling, Hepatitis C, VEGF Signaling
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